期刊
TALANTA
卷 89, 期 -, 页码 27-32出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2011.11.017
关键词
Electrochemiluminescence immunosensor; Alpha fetoprotein; Graphene-CdS QDs-agarose composites; Quantum dots; Graphene; Agarose
资金
- National Natural Science Foundation of China [81072336]
- 973 Program [2010CB635116]
- Science and Technology Department of Zhejiang Province of China [2011R405051]
- Ningbo Science and Technology Bureau [2010A610030, 2011C50037]
- Fund for Outstanding Young Teachers in Higher Education Institutions of Zhejiang Province
- Ningbo University [xk111049]
A novel strategy for the enhancement of electrochemiluminescence (ECL) was developed by combining CdS quantum dots (QDs), graphene (G) and agarose. This enhanced ECL was exploited to develop a label-free ECL immunosensor for the ultrasensitive detection of alpha fetoprotein (AFP). The novel G-CdS QDs-agarose composite was first coated on the glass carbon electrode surface to form a robust film, which exhibited high ECL intensity, good biocompatibility and high stability. After that 3-aminopropyltriethoxysilane (APS), as a binding linker, was conjugated to the G-CdS QDs-agarose composite film on the electrode, the ECL signal was significantly enhanced. The fabrication of ECL immunosensor was successfully completed by immobilizing the AFP-antibody (Ab) onto the electrode through glutaric dialdehyde (GLD). The specific immunoreaction between AFP and antibody resulted in the decrease in ECL intensity and the intensity decreased linearly with the logarithm of AFP concentration in the range of 0.0005-50 pg mL(-1) with a detection limit of 0.2 fg mL(-1). The immunosensor exhibits high sensitivity, specificity, stability, reproducibility and good regeneration, thus has the potential to be used in clinical application. Besides, the highly enhanced ECL from the G-CdS QDs-agarose composite film opened new avenues to apply graphene and QDs ECL in analytical systems and ECL biosensors. (C) 2011 Elsevier B.V. All rights reserved.
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