期刊
TALANTA
卷 88, 期 -, 页码 330-337出版社
ELSEVIER
DOI: 10.1016/j.talanta.2011.10.049
关键词
Ultrasound-assisted dispersive; liquid-liquid microextraction; High-performance liquid chromatography; Mequindox; Metabolite; Urine sample
资金
- Ministry of Science and Technology, P.R. China [2009CB118801]
- National Natural Science Foundation of China [20977112]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20090008120015]
- New Century Excellent Talents in University
A novel pretreatment method termed ultrasound-assisted dispersive liquid-liquid microextraction (UADLLME) coupled with high-performance liquid chromatography-ultraviolet detector (HPLC-UV) was applied for the detection of four synthesized metabolites of mequindox in pig urine samples. A total volume of 200 mu L of methanol (dispersant) and 60 mu L of 1,1,2,2-tetrachloroethane (extract) were injected into 5.0 mL of urine sample and then emulsified by ultrasound treatment for 4 min to form a cloudy solution. The effect of several factors on the recovery of each metabolite was investigated by a fitting derivation method for the first time. Under optimum conditions, the method yields a linear calibration curve in the concentration range from 0.5 to 500 mu g/L and a limit of detection (LOD) of 0.16-0.28 mu g/L for target analytes. The recoveries ranged from 72.0% to 91.3% with a relative standard deviation (RSD) of less than 5.2%. The enrichment factors for the four compounds ranged from 75 to 95. Two pig urine samples were successfully analyzed using the proposed method. (C) 2011 Elsevier B.V. All rights reserved.
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