期刊
TALANTA
卷 85, 期 3, 页码 1560-1565出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2011.06.048
关键词
Interdigitated arrays (IDA); Polyaniline-multiwalled carbon nanotube film (PANi-MWCNT); Peptide aptamer-antigen affinity; Electrochemical detection; Human papilloma virus (HPV)
资金
- Vietnam National Foundation for Science and Technology Development NAFOSTED [104.03-2010.60]
- MOST [59/2615/2010/HD-NDT]
In this study, polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825 Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150 kDa) on multifunctional PANi-MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen-antibody complex formation on well conducting IDA interface of PANi-MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works. (C) 2011 Elsevier B.V. All rights reserved.
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