期刊
TALANTA
卷 84, 期 1, 页码 78-83出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.12.034
关键词
Pyrolytic graphite electrode; Epinephrine; Norepinephrine; Smoking; Biological samples
资金
- Council of Scientific and Industrial research, New Delhi
- Department of Biotechnology, New Delhi
The simultaneous determination of catecholamines - epinephrine and norepinephrine by square wave voltammetry (SWV) at physiological pH 7.2 is reported using multi-walled carbon nanotubes modified edge plane pyrolytic graphite electrode (MWNT/EPPGE). A broad bump at similar to 250 mV is appeared for the oxidation of epinephrine (EP) and norepinephrine (NE) at bare EPPGE whereas at MWNT/EPPGE two well-separated peaks at similar to 150 and similar to 215 mV are appeared for the oxidation of EP and NE, respectively. The oxidation peak current of both the neurotransmitters also increased significantly along with the negative shift of peak potentials using MWNT/EPPGE. The oxidation of both compounds occurred in a pH dependent, 2e and 2H(+) process and the electrode reaction followed diffusion controlled pathway. Linear calibration curves were obtained for epinephrine and norepinephrine in the range 0.5-100 nM with limits of detection 0.15 x 10(-9) and 0.90 x 10(-10) M. respectively. The developed protocol is implemented for the simultaneous determination of epinephrine and norepinephrine in blood plasma and urine samples of smokers as well as in athletes. (C) 2010 Elsevier B.V. All rights reserved.
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