期刊
TALANTA
卷 84, 期 1, 页码 155-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.12.037
关键词
Flavonoids; Microemulsion electrokinetic chromatography; Mixed anionic and cationic surfactants; Radix Astragali; Sweeping
资金
- China Postdoctoral Science Foundation [20090461388]
In this report, a novel means for the separation and sweeping of flavonoids (quercetin, rutin, calycosin, ononin and calycosin-7-O-beta-D-glucoside) by microemulsion electrokinetic chromatography using mixed anionic and cationic surfactants as modified pseudostationary phase was presented. The optimized background electrolyte consisted of 0.5% (w/v) ethyl acetate, 2.0% (w/v) SOS, 9 mM DTAC, 4.0% (w/v) 1-butanol and 10 mM sodium borate or 25 mM phosphoric acid. We systematically investigated the separation and preconcentration conditions, including the concentrations of surfactant, types of sweeping, sample matrix, the effect of high salt or acetonitrile, and sample injection volume. It was found that the use of mixed surfactants significantly enhanced the separation efficiency through the change of the efficient electrophoretic mobility of analytes. Compared with normal sample injection, 185-508-fold sensitivity enhancement in terms of limit of detection was achieved through effective sweeping of large sample volume at 50 mbar pressure (up to 45% capillary length). At last, the proposed method was suitable for the determination of Radix Astragali sample. (C) 2011 Elsevier B.V. All rights reserved.
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