期刊
TALANTA
卷 83, 期 3, 页码 1050-1056出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.11.023
关键词
BODIPY-based derivative; Fluorescent probe; Cysteine detection; Human serum
资金
- NSF of China [20935005, 90813032, 20875092, 20905070]
- National Basic Research Program of China [2010CB933502, 2011CB935802]
- Chinese Academy of Sciences
A novel fluorescent probe is designed and synthesized for the determination of cysteine in biological samples by incorporating 2,4-dinitrobenzenesulfonyl (DBS) group as a quencher into the BODIPY skeleton. The BODIPY-based probe itself shows weak fluorescence due to the strong intramolecular charge transfer process. Upon reaction with cysteine, however, the probe produces a rapid and large fluorescence enhancement through the removal of the DBS unit by nucleophilic aromatic substitution. This valuable property leads to the development of a new and simple method for cysteine assay. Under the optimized conditions, the fluorescence enhancement value is directly proportional to the concentration of cysteine in the range 2-12 mu M, with a detection limit of 30 nM (S/N = 3). The applicability of the developed method has been successfully demonstrated on the determination of non-protein cysteine in human serum. Compared to most of the existing fluorescent probes proposed for cysteine, the BODIPY-based one exhibits an excellent overall performance in terms of selectivity, sensitivity and simplicity. (C) 2010 Elsevier B.V. All rights reserved.
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