期刊
TALANTA
卷 84, 期 4, 页码 1093-1098出版社
ELSEVIER
DOI: 10.1016/j.talanta.2011.03.021
关键词
Dimethylamine; Aliphatic amine; 1.3,5,7-Tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)-difluoroboradiaza-s-indacene (TMBB-Su); Fluorescence detection; High performance liquid chromatography
资金
- National Natural Science Foundation of China [20835004, 20775058, 30770550]
- Special Research Fund for the Doctoral Program of Higher Education of China [20070486031]
- Fundamental Research Funds for the Central Universities [1101004]
In this article, the simultaneous determination of primary and secondary aliphatic amines including dimethylamine. (DMA), diethylamine and eleven primary aliphatic amines by high performance liquid chromatography (HPLC) with fluorescence detection has been achieved using a BODIPY-based fluorescent derivatization reagent, 1,3,5,7-tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)-difluoroboradiaza-s-indacene (TMBB-Su). The derivatization reaction of TMBB-Su with aliphatic amities was optimized with orthogonal design experiment and the derivatization reaction proceeded at 15 C for 25 mm. The baseline separation of these derivatives was carried out on a C-8 column with methanol-tetrahydrofuran-50 mM pH 6.50 HAc-NaAc buffer (55/5/40, v/v/v) as a mobile phase. Detected at the excitation and emission of 490 and 510 nm, respectively, the detection limits were obtained in the range of 0.01-0.04 nM (signal-to-noise ratio = 3). The proposed method has been applied to the determination of trace aliphatic amines in viscera samples from mice without complex pretreatment or enrichment method. The recoveries ranged from 95.1% to 106.8%, depending on the samples investigated. (C) 2011 Elsevier B.V. All rights reserved.
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