期刊
TALANTA
卷 83, 期 1, 页码 185-189出版社
ELSEVIER
DOI: 10.1016/j.talanta.2010.09.004
关键词
Thrombin detection; Aptamer; Time resolved fluorescence; Eu(TTA)3(5-NH2-phen)
资金
- National Natural Science Foundation of China [20977026]
- National 863 High Technology Research Foundation of China [2006AA06Z407]
- Research Fund for the Doctoral Program of Higher Education of China [20090161110009]
- Equipment Foundation of Hunan University
- Changjiang Scholars and Innovative Research Team in University (PCSIRT)
In the present study the authors report a novel sensitive method for the detection of thrombin using time-resolved fluorescence sensing platform based on two different thrombin aptamers The thrombin 15-mer aptamer as a capture probe was covalently attached to the surface of glass slide and the thrombin 29-mer aptamer was fluorescently labeled as a detection probe A bifunctional europium complex was used as the fluorescent label The introduction of thrombin triggers the two different thrombin aptamers and thrombin to form a sandwich structure The fluorescence intensity is proportional to the thrombin concentration The present sensing system could provide both a wide linear dynamic range and a low detection limit The proposed sensing system also presented satisfactory specificity and selectivity Results showed that thrombin was retained at the aptamer-modified glass surface while nonspecific proteins were removed by rinsing with buffer solution This approach successfully showed the suitability of aptamers as low molecular weight receptors on glass slides for sensitive and specific protein detection (C) 2010 Elsevier B V All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据