期刊
TALANTA
卷 81, 期 1-2, 页码 505-509出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.12.031
关键词
Quantum dot; Aptamer; Probe; Label; Cell
资金
- National Key Scientific Program (973)-Nanoscience and Nanotechnology [2006CB933100]
- Program for New Century Excellent Talents in University [NCET-08-0046]
- National Natural Science Foundation of China [20505001]
- Excellent Young Scholars Research Fund of Beijing Institute of Technology [2007YS0603]
- Ministry of Public Health [2009ZX10004-107]
Fluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging. (C) 2009 Elsevier B.V. All rights reserved.
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