期刊
TALANTA
卷 82, 期 2, 页码 594-599出版社
ELSEVIER
DOI: 10.1016/j.talanta.2010.05.013
关键词
Extraction; Ultrasonic probe; 2-Mercaptoethanol; Biological samples; Mercury speciation
资金
- Spanish Government [CTQ-2008-05925, CTQ2008-01031-BQU]
- Ministerio de Medio Ambiente [046/PC08/2-14.4]
- Consejeria de Educacion de la Comunidad de Madrid
- European Social Fund [S-0505/AGR/0312]
- Ministerio de Ciencia e Innovacion
A fast method for mercury extraction from biological samples based on the use of HCl leaching plus different enzymatic hydrolysis (with and without mercury complexing agents), and the use of focussed ultrasounds (2-mm microtip) is here proposed. Total mercury content in several biological samples was determined by FI-ICP-MS using a carrier solution consisting of 0.1% (v/v) HCl, 0.1% (v/v) 2-mercaptoethanol, to avoid memory effect, and 0.15% (w/v) KCl. For mercury speciation a RP18 chromatographic column coupled to ICP-MS was used. A mobile phase consisting of 0.1% (v/v) formic acid, 0.1% (v/v) HFBA. 2% (v/v) methanol, and 0.02% (w/v) mM L-cysteine at pH 2.1 was used for chromatographic separation of the mercury species in the sample extracts. Extraction procedures were validated by using 50 mg of tuna fish tissue CRM-463 (2.85 +/- 0.16 mg kg(-1) for methylmercury). The recoveries obtained were 99 +/- 3% and 93 +/- 1% after acid leaching (HCl 7 M) and enzymatic extraction (15 mg protease type XIV in 2.5% (v/v) 2-mercaptoethanol). respectively. The optimal sonication conditions (5 min of exposure time and 40% of ultrasound amplitude) were applied to 5 mg of CRM-463 (88 +/- 5%), 5 mg of mussel tissue (81 +/- 11%) and to 2 mg of zebra fish embryos (90 +/- 10%) obtaining good recoveries in all cases. Methylmecury was found to be the most abundant Hg specie in all samples. The developed method is simple and rapid (5 min sample treatment); iris suitable for very small samples and does not alter the original form of the mercury species. Thus, it is of special interest in those cases in which validation of the results may often be hampered by lack of sample availability. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据