期刊
TALANTA
卷 82, 期 1, 页码 51-55出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.03.056
关键词
Resonance light scattering; CdTe QDs; Newcastle disease virus; Immunocomplex
资金
- National Natural Science Foundation of China [20475020, 20075009, 20875036]
- Ministry of Science and Technology of Jilin Province, China [20080544]
A sensitive QDs-based RLS assay method for the detection of Newcastle disease virus (NDV) antibody has been developed. CdTe quantum dots (QDs) were conjugated with Newcastle disease virus and used as RLS-based probes to detect NDV antibody. The electrostatic interaction between CdTe QDs and NDV resulted in enhanced resonance light scattering (RLS) signal characterized at 555 nm. Upon the addition of NDV antibody, QDs-NDV formed dispersive immunocomplex that can decrease the RLS signal. The decreased RLS intensity at 555 nm (Delta I-RLS) was linearly proportional to the concentration of NDV antibody (Canti-NDV) in the range of 0.5-50 ng/mL, with correlation coefficient of 0.974 and detection limit of 0.1 ng/mL under the optimization conditions. The proposed method was applied to the determination of NDV antibody in spiked samples with satisfactory results. (C) 2010 Elsevier B.V. All rights reserved.
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