期刊
TALANTA
卷 80, 期 5, 页码 1970-1979出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.10.056
关键词
UPLC; UHPLC; Tea; Phenolic compounds; Catechins; Flavonoids; Phenolic acids; Caffeine
资金
- Czech Ministry of Education [MSM 0021620822, 119308/8/2008]
Twenty nine phenolic compounds comprising nine phenolic acids, sixteen flavonoids (including eight tea catechins, glycosides and aglycones), four coumarins plus caffeine were analysed within 20 min using ultra high performance liquid chromatography (UHPLC) with PDA detection. UHPLC system was equipped with C18 analytical column (100 mm x 2.1 mm, 1.7 mu m), utilising 0.1% formic acid and methanol mobile phase in the gradient elution mode The developed method was tested for the system suitability resolution, asymmetry factor, peak capacity, retention time repeatability and peak area repeatability. The method was fully validated in the terms of linearity (r(2) > 0.9990 for all 30 compounds), range (typically 1-100 mg L(-1)). LOD, LOQ inter/intra-day precision (<3% and <9% respectively) and inter/intra-day accuracy (typically 100 +/- 10%). Subsequently the method was applied to the identification (spectral information and peak purity calculations were profited) and quantification of phenolic compounds and caffeine present in tea infusions and extracts (C) 2009 Elsevier B.V All rights reserved.
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