期刊
TALANTA
卷 82, 期 3, 页码 997-1002出版社
ELSEVIER
DOI: 10.1016/j.talanta.2010.06.005
关键词
Quantum dots; Sensor; Glucose; Glucose oxidase; Hydrogen peroxide; Photoluminescence
资金
- National Natural Science Foundation of China [30425020, 90813010]
- Major National Science and Technology Projects [20092ZX10004-301]
- Shanghai Pujiang Program [081114011]
- Shanghai Leading Academic Discipline Project [B113]
A novel label-free detection system based on CdTe/CdS quantum dots (QDs) was designed for the direct measurement of glucose. Herein we demonstrated that the photoluminescence (PL) of CdTe/CdS QDs was sensitive to hydrogen peroxide (H2O2). With D-glucose as a substrate, H2O2 that intensively quenched the QDs PL can be produced via the catalysis of glucose oxidase (GOx). Experimental results showed that the decrease of the QDs PL was proportional to the concentration of glucose within the range of 1.8 mu M to 1 mM with the detection limit of 1.8 mu M under the optimized experimental conditions. In addition, the QD-based label-free glucose sensing platform was adapted to 96-well plates for fluorescent assay, enhancing the capabilities and conveniences of this detection platform. An excellent response to the concentrations of glucose was found within the range of 2-30 mM. Glucose in blood and urine samples was effectively detected via this strategy. The comparison with commercialized glucose meter indicated that this proposed glucose assay system is not only simple, sensitive, but also reliable and suitable for practical application. The high sensitivity, versatility, portability, high-throughput and low cost of this glucose sensor implied its potential in point-of-care clinical diagnose of diabetes and other fields. (C) 2010 Elsevier B.V. All rights reserved.
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