期刊
TALANTA
卷 82, 期 2, 页码 483-487出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.04.061
关键词
Choline biosensor; Supramolecular complex; beta-Cyclodextrin; Platinum nanoparticles; Square wave voltammetry
资金
- National Natural Science Foundation of China [90713022]
- National Key Basic Research Development Project of China [2007CB714500]
- Chinese Academy of Sciences [KJCX2-YW-H11]
A sensitive biosensor with supramolecular architecture was designed and implemented here to detect choline. Choline oxidase and horseradish peroxidase were assembled onto the polymer of thiolated beta-cyclodextrin and platinum nanoparticles modified gold electrode through 1-adamantane carboxylic acid coupling. Square wave voltammetry showed that the reduction currents at 0.38 mV had a linear relationship with the logarithms of choline concentrations in the range of 10(-9)-10(-2) M. and the detection limit was down to 0.1 nM. Such biosensor also exhibited excellent selectivity, reproducibility and stability. (C) 2010 Elsevier B.V. All rights reserved.
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