期刊
TALANTA
卷 80, 期 2, 页码 607-613出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.07.034
关键词
Fluorescent probe; Triphenylmethane dye; G-quadruplex; Double-stranded DNA; Single-stranded DNA
资金
- National Natural Science Foundation of China [20801040]
- National Basic Research Program of China [2006CB705700]
- Natural Science Foundation of Tianjin [08JCZDJC21200]
Triphenylmethane (TPM) dyes normally render rather weak fluorescence due to easy vibrational deexcitation. However, when they stack onto the two external G-quartets of a G-quadruplex (especially intramolecular G-quadruplex), such vibrations will be restricted, resulting in greatly enhanced fluorescence intensities. Thus, TPM dyes may be developed as sensitive G-quadruplex fluorescent probes. Here, fluorescence spectra and energy transfer spectra of five TPM dyes in the presence of G-quadruplexes, single- or double-stranded DNAs were compared. The results show that the fluorescence spectra of four TPM dyes can be used to discriminate intramolecular G-quadruplexes from intermolecular G-quadruplexes, single- and double-stranded DNAs. The energy transfer fluorescence spectra and energy transfer fluorescence titration can be used to distinguish G-quadruplexes (including intramolecular and intermolecular G-quadruplexes) from single- and double-stranded DNAs. Positive charges and substituent size in TPM dyes may be two important factors in influencing the binding stability of the dyes and G-quadruplexes. (C) 2009 Elsevier B.V. All rights reserved.
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