期刊
TALANTA
卷 80, 期 2, 页码 853-857出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.08.003
关键词
Biosensor; Optical fiber; Laccase; Catecholamines; Urine; Plasma
资金
- FCT (Portugal) [PTDC/QUI/70970/2006]
- Development of a new Optical Fiber Biosensor for Determination of Catecholamines (CATSENSOR) [SFRH/BD/44175/2008]
- Fundação para a Ciência e a Tecnologia [PTDC/QUI/70970/2006] Funding Source: FCT
An optical fiber biosensor has been developed for the determination of catecholamines (dopamine, norepinephrine and epinephrine) based on the recognition capacity of the enzyme laccase. In this study, a glass tube constituted by a fused silica fiber coated with a film of polystyrene/divinylbenzene resin (PS/DVB) was used for catecholamines separation. Firstly, the analyzer was tested for calibration and its analytical performance for catecholamines detection was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED). The developed analytical device shows a high potential for catecholamines quantification with a detection limit of 2.1, 2.6 and 3.4 pg mL(-1) for dopamine, norepinephrine and epinephrine, respectively. The analytical sensitivity, inferred from the slope of the calibration curves established for a range of concentrations between 5 and 125 pg mL(-1), was found to be 0.344, 0.252 and 0.140 dB/pg mL(-1) for dopamine, norepinephrine and epinephrine, respectively. Furthermore, catecholamines speciation with the PS/DVB fiber was completely achieved in 3 min. The analytical performance of the reported sensor was also evaluated and found adequate for catecholamines determination in human urine and plasma samples. (C) 2009 Elsevier B.V. All rights reserved.
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