期刊
TALANTA
卷 79, 期 2, 页码 254-259出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.03.045
关键词
Metabolomics; Ultra-performance liquid chromatography/time-of-flight mass; spectrometry (UPLC/TOF-MS); Multivariate analysis; Myocardial infarction; Lipid biomarkers
资金
- National Key Technology RD Program [2006BAI081304-01]
- National Natural Science Foundations of China [20805026]
An ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOF-MS)-based metabolomic approach was developed to characterize the metabolic profile associated with isoproterenol (ISO)-induced myocardial infarction (MI). Analysis of the serum samples revealed distinct changes in the biochemical patterns of ISO-induced rats. A multivariate statistical method, supervised partial least squares-discriminant analysis (PLS-DA), was then used for screening of potential biomarkers. As a result, 13 lipid biomarkers, including lysophosphatidylcholines (Lyso-PCs) and fatty acids were identified by the accurate mass measurement of TOF-MS. The relationship between abnormal lipid metabolism and the formation of MI were also studied. This work demonstrates the utility of UPLC/TOF-MS-based metabolic profiling combined with multivariate analysis as a powerful tool to further investigate the pathogenesis of cardiovascular diseases. (C) 2009 Elsevier B.V. All rights reserved.
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