期刊
TALANTA
卷 78, 期 3, 页码 1023-1028出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.01.008
关键词
Co-immobilised glucose oxidase and hexakinase; Platinum microelectrode; Amperometric microbiosensor; ATP-analysis; Reproducibility; Operational and storage stability
资金
- Claude Bernard University of Lyon (France)
- National Academy of Sciences of Ukraine
- NATO Collaborative Linkage Grant [CBP.NUKR.CLG 982788]
ATP determination is of great importance since this compound is involved in a number of vital biological processes. To monitor ATP concentration levels, we have developed a microbiosensor based on cylindrical platinum microelectrode, covered with a layer of poly-m-phenylendiamine (PPD), and layer of co-immobilised glucose oxidase and hexokinase. Conditions for biosensor measurement of ATP (pH, Mg2+ and substrates concentration) in vitro and microbiosensor characteristics such as sensitivity, selectivity, reproducibility. storage stability were studied and optimized. Under optimal conditions the microbiosensor can measure ATP concentrations down to a 2.5 mu M detection limit with response time about 15 s. Interferences by electroactive compounds like biogenic amines and their metabolites, ascorbic acid, uric acid and L-cystein are rejected in general by the PPD layer. The microbiosensor developed is insensitive to ATP analogues (or substances with similar structure), such as ADP, AMP, GTP and UTP, too. It can be used for ATP analysis in vitro in the reactions consuming or producing macroergic triphosphate molecules to study kinetics of the process and in drug design concerning development of inhibitors specific to target kinases and others target enzymes. (c) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据