期刊
TALANTA
卷 79, 期 1, 页码 103-108出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.03.018
关键词
Ruthenium complex; Luminescent nanoparticles; Bioimaging; Time-gated luminescence; Environmental pathogen
资金
- National Natural Science Foundation of China [20575069, 20835001]
- Higher Education of China [200801410003]
- Macquarie University Research Fellowship Scheme
Luminescent ruthenium(II) complex covalently bound silica nanoparticles have been prepared and used as a probe for time-gated luminescence bioimaging. The new nanoparticles were prepared by copolymerization of a luminescent Ru(II) complex tris(5-amino-1,10-phenanthroline)ruthenium(II) conjugated with 3-aminopropyl(triethoxy)si lane (APS-Ru conjugate), free (3 -aminopropyl)triethoxysilane (APS) and tetraethyl orthosilicate (TEOS) in a water-in-oil reverse microemulsion consisting of Triton X-100, n-octanol, cyclohexane and water in the presence of aqueous ammonia. Characterization by transmission electron microscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, 64 +/- 4 nm in diameter. Compared with the dye-doping nanoparticles, dye leakage of the new nanoparticles was remarkably decreased. In addition, it was found that the Ru(II) complex luminescence could be effectively enhanced with a longer luminescence lifetime (similar to 2.3 mu s) after forming the nanoparticles, which enables the nanoparticles to be suitable as a bioprobe for time-gated luminescence bioimaging applications. The nanoparticle-labeled streptavidin was prepared and successfully used for time-gated luminescence imaging detection of an environmental pathogen, Giardia lamblia, with high specificity and sensitivity. (C) 2009 Elsevier B.V. All rights reserved.
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