期刊
TALANTA
卷 79, 期 2, 页码 493-498出版社
ELSEVIER
DOI: 10.1016/j.talanta.2009.04.026
关键词
Phytochelatins; HPLC-ICP-MS; HPLC-ESI-MSn; Pisum sativum
资金
- KBN [1 T09D 05730]
An analytical approach based on hyphenated techniques was used for studying the speciation of cadmium and lead in Pisum sativum. Proper preservation conditions were employed to avoid the oxidation of -SH groups and corresponding decomposition of metal-binding complexes. SEC column was washed with 5 mM beta-mercaptoethanol and then samples were analysed using ICP-MS as a detector. Results showed that cadmium is the inhibitor of lead uptake. HPLC-ESI-MSn assays revealed fragmentation pathways of phytochelatins. (C) 2009 Elsevier B.V. All rights reserved.
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