期刊
TALANTA
卷 78, 期 3, 页码 1141-1147出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2009.01.037
关键词
Aristolochic acid I; High-performance liquid chromatography; Determination; L-02 cell; Cell toxicology
资金
- National Basic Research Program [2006CB504701]
- National Natural Science Foundation of China [20575019]
- Jiangxi Province Office of Education [GJJ09277]
Aristolochic acid I (AA I), a major component of the carcinogenic plant extract aristolochic acid (AA), is known to be nephrotoxic, carcinogenic and mutagenic. A simple, rapid and sensitive high-performance liquid chromatography-diode array detection-fluorescence detection (HPLC-DAD-FLD) method was developed and validated for the analysis of AA I and its metabolites in cell culture medium for the first time. The samples were prepared with ethyl acetate liquid-liquid extraction (LLE). Good separation was obtained on an ODS C-18 analytical column with 0.2% HAc/methanol gradient solution. Linearities of about three orders of magnitude were gained with cot-relation coefficients exceeding 0.9990. The method appears to be a suitable tool for the cellular toxicokinetic study with acceptable precisions and recoveries. Cytotoxicity of AA I on human liver cells (L-02) was investigated with morphological observation and MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay, cytotoxicity increased in AA I concentration-dependent manner. AA I and its metabolites were monitored with the proposed chromatographic analysis, and some preliminary toxicokinetics were investigated. (c) 2009 Elsevier B.V. All rights reserved.
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