期刊
TALANTA
卷 77, 期 2, 页码 606-623出版社
ELSEVIER
DOI: 10.1016/j.talanta.2008.07.024
关键词
DNA biosensor review; DNA chip; Lab-on-a-chip; Molecular diagnosis; Nanobiotechnology
资金
- FCT (Fundacao Para a Ciencia e Tecnologia), Portugal [PTDC/AMB/73154/2006]
- Fundação para a Ciência e a Tecnologia [PTDC/AMB/73154/2006] Funding Source: FCT
Biosensors have witnessed at) escalating interest nowadays, both in the research and commercial fields. Deoxyribonucleic acid (DNA) biosensors (genosensors) have been exploited for their inherent physico-chemical stability and suitability to discriminate different organism strains. The main principle of detection among genosensors relies on specific DNA hybridization, directly on the surface of a physical transducer. This review covers the main DNA immobilization techniques reported so far, new micro-and nanotechnological platforms for biosensing and the transduction mechanisms in genosensors. Clinical applications, in particular, demand large-scale and decentralized DNA testing. New schemes for DNA diagnosis include DNA chips and microfluidics, which couples DNA detection with sample pretreatment under in vivo-like hybridization conditions. Higher sensitivity and specificity may arise from nanoengineered structures, like carbon nanotubes(CNTs) and DNA/protein conjugates. A new platform for Universal DNA biosensing is also presented, and its implications for the future of molecular diagnosis are argued. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据