The application of CdTe@SiO2 particles in immunoassay

CdTe@SiO2 fluorescent particles were synthesized via hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in water-in-oil (W/O) emulsion. Uniform luminophore-doped silica nanoparticles with 100 nm in diameter were obtained using microemulsion method and characterized by SEM. Antibody proteins were successfully conjugated to the fluorescent particles by the reaction of avidin and biotin, which were confirmed by fluorescence spectra. CdTe@SiO2 fluorescent particles were potentially useful for the applications in biolabeling and imaging. (C) 2008 Elsevier B.V. All rights reserved.