期刊
TALANTA
卷 76, 期 4, 页码 791-797出版社
ELSEVIER
DOI: 10.1016/j.talanta.2008.04.027
关键词
biogenic amines; derivatization; food samples; 3-(4-fluorobenzoyl)-2-quinolinecarboxaldehyde (FBQCA); micellar electrokinetic capillary chromatography laser-induced fluorescence
资金
- National Natural Science Foundation of China [20575047, 30770550, 20775058]
An effective approach was proposed to the derivatization of seven biogenic amines using 3-(4-fluorobenzoyl)-2-quinolinecarboxaldehyde (FBQCA) as a fluorogenic reagent. The sensitive determinations of these derivatives were achieved by micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence (LIF) detection. The derivatization and electrophoretic conditions have been optimized. A running buffer was composed of mixtures of 25 mM pH 9.5 boric acid, 25 mM SDS, and 27% ACN. At 25 degrees C and 22.5 kV, the baseline separation of the derivatives was accomplished in 13 min. The detection limit (S/N =3) was found as low as 0.4 nM. The proposed method was validated by the linearity of two orders magnitude and correlation coefficient in the range 0.9969-0.9998. Also, the procedure was successfully applied to the determination of biogenic amines in SOY Sauce, fish and wine samples. (C) 2008 Published by Elsevier B.V.
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