期刊
TALANTA
卷 76, 期 2, 页码 458-461出版社
ELSEVIER
DOI: 10.1016/j.talanta.2008.03.038
关键词
molecular beacon; real-time monitoring; restriction endonuclease; DNA cleavage
Traditional methods to assay enzymatic cleavage of DNA are discontinuous, time-consuming and laborious. Here, we report a new approach for real-time monitoring of double-stranded DNA cleavage by restriction endonuclease based on nucleic acid ligation using molecular beacon. Upon cleavage of DNA, the cleavage product can be ligated by DNA ligase, which results in a fluorescence enhancement of the molecular beacon. This method permits real-time monitoring of DNA cleavage and makes it easy to characterize the activity of restriction enclonuclease and to study the cleavage reaction kinetics. (C) 2008 Elsevier B.V. All rights reserved.
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