期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 37, 期 7, 页码 467-473出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.syapm.2014.07.001
关键词
rpoB; gyrB; 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS
资金
- Broad Institute Genome Sequencing Platform within the collaborative project (NCBI BioProject) by Patrice Courvalin (Institut Pasteur, Paris) [PRJNA183623]
- Czech Science Foundation [13-26693S]
We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (<= 77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate L-histidine and the inability to grow at 35 degrees C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T) = CCUG 63842(T) = CCM 8462(T)). (C) 2014 Elsevier GmbH. All rights reserved.
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