期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 35, 期 5, 页码 302-305出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2012.06.001
关键词
Bradyrhizobium; Cytisus; Phylogeny; Morocco
资金
- Junta de Castilla y Leon (Regional Spanish Government)
- MICINN (Central Spanish Government)
- ERDF
- Consejeria de Economia, Innovacion y Ciencia (Junta de Andalucia, Spain) [RNM-4746]
- CSIC-CNRST [2009MA0013]
- JAE-Doc researcher contract from CSIC
Two bradyrhizobial strains, CTAW71(T) and CTAW69, previously isolated from root nodules of Cytisus villosus, have been analysed using a polyphasic approach. These strains have identical 16S rRNA genes and their closest relative species is Bradyrhizobium cytisi, whose type strain CTAW11(T) presented 99.8% identity with respect to strain CTAW71(T). Despite the closeness of the 16S rRNA genes, the housekeeping genes recA, atpD and glnII harboured by strain CTAW71(T) were divergent to those from B. cytisi CTAW11(T), with identity values of 93%, 95% and 97%, respectively. These differences were congruent with DNA-DNA hybridization analysis that revealed an average of 37% relatedness between strain CTAW71(T) and B. cytisi CTAW11(T). Phenotypic characteristics were identical for strains CTAW71(T) and CTAW69, but differed from those of the described species from genus Bradyrhizobium. Based on the genotypic and phenotypic data obtained in this study, we propose that strains CTAW71(T) and CTAW69 should be classified into a new species for which the name Bradyrhizobium rifense sp. nov. is proposed (type strain CTAW71(T) = LMG 26781(T) = CECT 8066(T)). (c) 2012 Published by Elsevier GmbH.
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