期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 31, 期 1, 页码 24-29出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.syapm.2007.10.003
关键词
Nocardioides halotolerans sp nov.; polyphasic taxonomy; 16S rRNA
A strictly aerobic, Gram-positive, motile, coccoid-shaped, halotolerant actinobacterium (10% NaCl, w/v), designated MSL-23(T), was isolated from a soil sample on Bigeum Island, Korea. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Nocardioides, with the highest sequence similarity (95.63%) being to Nocardioides kribbensis KCTC 19038(T). The major menaquinone was MK-8(H-4), and the predominant cellular fatty acids were i-C-16:0, ai-C-17:0, C-18:1 omega 9c and 10-methyl-C-16:0. The DNA G+C content was 69.7mol%. The 16S rRNA gene sequence of strain MSL 23(T) and its chemotaxonomic properties showed it to be unique in the genus Nocardioides. Phenotypic characteristics distinguished strain MSL-23(T) from other Nocardioides species. On the basis of the phenotypic, chemotaxonomic and phylogenetic data strain MSL-23(T) represents a novel species, for which the name Nocardioides halotolerans sp. nov. is proposed, with MSL-23(T) (= KCTC 19274(T) = DSM 19273(T)) as the type strain. (C) 2007 Elsevier GmbH. All rights reserved.
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