A novel hydrogen peroxide biosensor based on the specific binding of horseradish peroxidase with polymeric thiophene-3-boronic acid monolayer in hydrophilic room temperature ionic liquid

In the present work, we have developed a novel hydrogen peroxide (H2O2) biosensor based on electropolymerization of thiophene-3-boronic acid film (TBA) in ionic liquid via glucosidic bond with glycoprotein horseradish peroxidase (HRP). A thin film of poly (thiophene-3-boronic acid) (PTBA) has been synthesized by electropolymerization using room temperature ionic liquids (RTIL) as the growth medium and the supporting electrolyte. Horseradish peroxidase (HRP) in this study shows affinity interaction with the formed PTBA thin film. With the aid of hydroquinone (H(2)Q) mediator, the biosensor has a fast response of less than 7s with linear range of 5.0 x 10(-6) to 1.75 x 10(-4) M and a detection limit of 0.5 mu M. The developed biosensor exhibits fast response, a low detection limit, high stability and very good reproducibility. (C) 2011 Elsevier B.V. All rights reserved.