期刊
SYNTHETIC METALS
卷 159, 期 21-22, 页码 2159-2161出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.synthmet.2009.08.024
关键词
Cytochrome c; Poly(5-amino-1-naphthol); Nitric oxide detection
A nitric oxide biosensor based on cytochrome c (an heme protein) covalently immobilized to poly(5-amino-1-naphthol) by using cyanuric chloride as a bridge was developed. The immobilization was studied by cyclic voltammetry and quartz crystal microbalance. The nitric oxide detection as a function of poly(5-amino-1-naphthol) amount was recorded, and the best result was obtained with the electrode prepared by 70 cycles. The sensitivity and detection limit were 0.015 mu A cm(-2)/mu mol L-1 and 2.85 mu mol L-1, respectively. (C) 2009 Elsevier B.V. All rights reserved.
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