Stress-induced synaptic changes in the rat anterior cingulate cortex are dependent on endocrine developmental time windows

Fetal and neonatal brain development is characterized by developmental time windows during which brain regions or neuron types are specifically sensitive to environmental influences. Previous studies on cortical development have revealed evidence for the hypothesis that the extent and the direction of experience-induced neuronal and synaptic changes correlate with time windows of endocrine development. To further test this hypothesis we exposed rats to neonatal separation stress during different phases of endocrine maturation, i.e. prior, during and after the stress hyporesponsive period (SHRP) of the hypothalamic-pituitary-adrenal (HPA) axis. We show here that only stress during the SHRP resulted in significantly decreased (-29%) spines densities on the basal dendrites of pyramidal cells in layer V of the anterior cingulate cortex (ACd), whereas stress during the other two tested time windows had no effect on these parameters. Dendritic length remained unaffected by stress exposure at any of the tested time windows. These results reveal specific developmental time window for synaptic wiring within the deeper layers of the anterior cingulate cortex, which seem not to be mediated by hormonally induced mechanisms.