期刊
SYMBIOSIS
卷 48, 期 1-3, 页码 120-130出版社
SPRINGER
DOI: 10.1007/BF03179991
关键词
Bacterial fingerprinting; bacterial taxonomy; biological nitrogen fixation; Rhizobiales; inoculants; 16S rRNA
类别
With more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N-2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and 16S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach - 20%:80% of BOX-PCR:16S rRNA which correlated well with the 16S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups - might expedite rhizobial diversity studies.
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