期刊
SURGERY
卷 148, 期 2, 页码 319-324出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.surg.2010.04.025
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类别
资金
- National Cancel Institute (NCI) [1U54CA143837-01, CA16672]
- Kanmus Foundation
- National Institutes of Health (NIH) [5 T32 CA09599, T32]
Background. Cold and carbon nanoparticles absorb nonionizing radio fiequency (RF) energy and release heat. Solid gold nanoparticles are delivered to cancer cells via conjugation with targeting antibodies Here, 20-nm gold particles were conjugated to centuximab which is an epidermal growth factor receptor-1 (EGER-I) antibody. Methods. A pancreatic carcinoma cell line that highly expresses EGFR-1, Panc-1, and Cama-1, which is a breast carcinoma cell line that minimally expresses EGFR-1, were treated with 100-nmol/L cetuximab-conjugated gold nanoparticles for 3 h (n = 4). Thirty-six hours later; the dishes were placed in an RF field with a generator power of 200 W for 5 min After another 36 h, cell injury and death were evaluated with flow cytometry. Results. The targeted cell line Panc-1 had a viability of 46% 12%, whereas the Cama-1 cell had a viability of 92% +/- 2% after RF field expovine (P < 008). Transmission electron microscopy showed gold nanoparticle uptake in Panc-1 cells but. negligible uptake by Cama-1 cells Nontargeted cells do not internalize a sufficient amount of antibody-conjugated gold nanoparticles to induce injury in. a noninvasive RE field Conclusion. This technique could be useful in cancer treatment if a cancer-specific antibody is used to localize gold nanoparticles to malignant cells (Surgery 2010,148:319-24)
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