期刊
SURFACE AND INTERFACE ANALYSIS
卷 43, 期 1-2, 页码 261-264出版社
WILEY-BLACKWELL
DOI: 10.1002/sia.3537
关键词
ToF-SIMS imaging; principal component analysis; image contrast; depth profile; surface analysis
资金
- NIH [EB-002027, EB-001473]
Imaging time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been used to study protein bound to a photolithographically patterned, commercial poly(ethylene glycol) (PEG)-based polymer film. The effect of different ion sources on the fragmentation pattern from this sample was analyzed with respect to the surface sensitivity of characteristic protein fragments and contrast in the ion images. The method demonstrates that, under similar fluence (below the static limit), Bi-3(+) provides better surface sensitivity for low mass fragments, and the best image contrast, as compared to Bi-1(+) and C-60(+) ion sources. Principal component analysis (PCA) was utilized to process depth profiles for this sample and shows that a primary ion fluence of approximately 20 x 10(12) ions/cm(2) is required to etch through the adsorbed protein layer. Copyright (C) 2010 John Wiley & Sons, Ltd.
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