期刊
STUDIES IN MYCOLOGY
卷 -, 期 74, 页码 47-57出版社
CENTRAALBUREAU SCHIMMELCULTURE
DOI: 10.3114/sim0008
关键词
Aspergillus; bioreactor; colony; heterogeneity; mycelium
类别
资金
- IOP Genomics program of the Dutch Ministry of Economic Affairs
Black pigmented conidia of Aspergillus niger give rise to micro-colonies when incubated in liquid shaken medium. These micro-colonies are heterogeneous with respect to gene expression and size. We here studied the biophysical properties of the conidia of a control strain and of strains in which the fwnA, olvA or brnA gene is inactivated. These strains form fawn-, olive-, and brown-coloured conidia, respectively. The Delta olvA strain produced larger conidia (3.8 mu m) when compared to the other strains (3.2-3.3 mu m). Moreover, the conidia of the Delta olvA strain were highly hydrophilic, whereas those of the other strains were hydrophobic. The zeta potential of the Delta olvA conidia in medium was also more negative when compared to the control strain. This was accompanied by the near absence of a rodlet layer of hydrophobins. Using the Complex Object Parametric Analyzer and Sorter it was shown that the ratio of individual hyphae and micro-colonies in liquid shaken cultures of the deletion strains was lower when compared to the control strain. The average size of the micro-colonies of the control strain was also smaller (628 mu m) than that of the deletion strains (790-858 mu m). The size distribution of the micro-colonies of the Delta fwnA strain was normally distributed, while that of the other strains could be explained by assuming a population of small and a population of large micro-colonies. In the last set of experiments it was shown that relative expression levels of gpdA, and AmyR and XlnR regulated genes correlate in individual hyphae at the periphery of micro-colonies. This indicates the existence of transcriptionally and translationally highly active and lowly active hyphae as was previously shown in macro-colonies. However, the existence of distinct populations of hyphae with high and low transcriptional and translational activity seems to be less robust when compared to macro-colonies grown on solid medium.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据