期刊
STRUCTURE
卷 22, 期 6, 页码 878-888出版社
CELL PRESS
DOI: 10.1016/j.str.2014.04.010
关键词
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资金
- Canadian Institutes of Health Research (CIHR) [MOP-48370, MOP-14606]
- Canadian Foundation for Innovation
- CIHR training related to synchrotron techniques (CIHR-TRUST) fellowship
Shigella invasion of its human host is assisted by T3SS-delivered effector proteins. The OspG effector kinase binds ubiquitin and ubiquitin-loaded E2-conjugating enzymes, including UbcH5b and UbcH7, and attenuates the host innate immune NF-kB signaling. We present the structure of OspG bound to the UbcH7 similar to Ub conjugate. OspG has a minimal kinase fold lacking the activation loop of regulatory kinases. UbcH7 similar to Ub binds OspG at sites remote from the kinase active site, yet increases its kinase activity. The ubiquitin is positioned in the open'' conformation with respect to UbcH7 using its I44 patch to interact with the C terminus of OspG. UbcH7 binds to OspG using two conserved loops essential for E3 ligase recruitment. The interaction of the UbcH7 similar to Ub with OspG is remarkably similar to the interaction of an E2 similar to Ub with a HECT E3 ligase. OspGinterferes with the interaction of UbcH7 with the E3 parkin and inhibits the activity of the E3.
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