期刊
STRUCTURE
卷 20, 期 4, 页码 582-592出版社
CELL PRESS
DOI: 10.1016/j.str.2012.02.017
关键词
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资金
- NIH [5R01GM098672, 1S10RR026814-01]
- UCSF
- German Research Foundation [SFB 807, TA157/7]
- NIGMS-IMSD
- HARC Center [P50GM082250]
- Membrane Protein Expression Center [1P50 GM073210]
In spite of its recent achievements, the technique of single particle electron cryomicroscopy (cryoEM) has not been widely used to study proteins smaller than 100 kDa, although it is a highly desirable application of this technique. One fundamental limitation is that images of small proteins embedded in vitreous ice do not contain adequate features for accurate image alignment. We describe a general strategy to overcome this limitation by selecting a fragment antigen binding (Fab) to form a stable and rigid complex with a target protein, thus providing a defined feature for accurate image alignment. Using this approach, we determined a three-dimensional structure of an similar to 65 kDa protein by single particle cryoEM. Because Fabs can be readily generated against a wide range of proteins by phage display, this approach is generally applicable to study many small proteins by single particle cryoEM.
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