期刊
STRUCTURE
卷 20, 期 6, 页码 967-976出版社
CELL PRESS
DOI: 10.1016/j.str.2012.04.010
关键词
-
资金
- NIH [P50 GM073197]
- NCRR
- NIGMS [R21 GM103972]
Structural studies of human G protein-coupled receptors (GPCRs) have recently been accelerated through the use of a fusion partner that was inserted into the third intracellular loop. Using chimeras of the human beta(2)-adrenergic and human A(2A) adenosine receptors, we present the methodology and data for the initial selection of an expanded set of fusion partners for crystallizing GPCRs. In particular, use of the thermostabilized apocytochrome b(562)RIL as a fusion partner displays certain advantages over previously utilized fusion proteins, resulting in a significant improvement in stability and structure of GPCR-fusion constructs.
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