期刊
STRUCTURE
卷 20, 期 11, 页码 1940-1947出版社
CELL PRESS
DOI: 10.1016/j.str.2012.08.027
关键词
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资金
- Office of Biological and Environmental Research of the U.S. Department of Energy
- Office of Basic Energy Sciences of the U.S. Department of Energy
- National Center for Research Resources of the National Institutes of Health (NIH)
- NIH [R00-ES015421, R01-GM100021]
- United Mitochondrial Disease Foundation [59042]
MTERF4 is the first MTERF family member shown to bind RNA and plays an essential role as a regulator of ribosomal biogenesis in mammalian mitochondria. It forms a complex with the rRNA methyltransferase NSUN4 and recruits it to the large ribosomal subunit. In this article, we characterize the interaction between both proteins, demonstrate that MTERF4 strongly stimulates the specificity of NSUN4 during in vitro methylation experiments, and present the 2.0 angstrom resolution crystal structure of the MTERF4: NSUN4 protein complex, lacking 48 residues of the MTERF4 C-terminal acidic tail, bound to S-adenosyl-L-methionine, thus revealing the nature of the interaction between both proteins and the structural conservation of the most divergent of the human MTERF family members. Moreover, the structure suggests a model for RNA binding by the MTERF4: NSUN4 complex, providing insight into the mechanism by which an MTERF family member facilitates rRNA methylation.
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