期刊
CELL REPORTS
卷 10, 期 10, 页码 1749-1757出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.02.028
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资金
- Novo Nordisk Foundation
- Danish Cancer Society
- Lundbeck Foundation
- Association for International Cancer Research
- European Research Council
- Nordea Foundation
- European Commission project DDResponse, Czech national program of sustainability [LO1304]
- Czech Ministry of Interior [VG20102014001]
- Villum Kann Rasmussen Fund
- Danish National Research Foundation
- Danish Medical Research Council
- Swiss National Science Foundation
- Krebsliga Schweiz
- Novo Nordisk Fonden [NNF12OC0002360] Funding Source: researchfish
DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal. Here, we show that FBH1 catalyzes regression of a model replication fork in vitro and promotes fork regression in vivo in response to replication perturbation. Cells respond to fork stalling by activating checkpoint responses requiring signaling through stress-activated protein kinases. Importantly, we show that FBH1, through its helicase activity, is required for early phosphorylation of ATM substrates such as CHK2 and CtIP as well as hyperphosphorylation of RPA. These phosphorylations occur prior to apparent DNA double-strand break formation. Furthermore, FBH1-dependent signaling promotes checkpoint control and preserves genome integrity. We propose a model whereby FBH1 promotes early checkpoint signaling by remodeling of stalled DNA replication forks.
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