期刊
CELL REPORTS
卷 10, 期 3, 页码 398-413出版社
CELL PRESS
DOI: 10.1016/j.celrep.2014.12.037
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资金
- Cancer Research UK
- Breast Cancer Campaign
- West of Scotland Women's Bowling Association
- Cancer Research UK [15673, 18277, 15565] Funding Source: researchfish
- Chief Scientist Office [CAF/06/24] Funding Source: researchfish
Integrin trafficking is key to cell migration, but little is known about the spatiotemporal organization of integrin endocytosis. Here, we show that alpha 5 beta 1 integrin undergoes tensin-dependent centripetal movement from the cell periphery to populate adhesions located under the nucleus. From here, ligand-engaged alpha 5 beta 1 integrins are internalized under control of the Arf subfamily GTPase, Arf4, and are trafficked to nearby late endosomes/lysosomes. Suppression of centripetal movement or Arf4-dependent endocytosis disrupts flow of ligand-bound integrins to late endosomes/lysosomes and their degradation within this compartment. Arf4-dependent integrin internalization is required for proper lysosome positioning and for recruitment and activation of mTOR at this cellular subcompartment. Furthermore, nutrient depletion promotes subnuclear accumulation and endocytosis of ligand-engaged alpha 5 beta 1 integrins via inhibition of mTORC1. This two-way regulatory interaction between mTORC1 and integrin trafficking in combination with data describing a role for tensin in invasive cell migration indicate interesting links between nutrient signaling and metastasis.
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