期刊
CELL REPORTS
卷 13, 期 4, 页码 798-811出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.09.041
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资金
- Natural Science Foundation of China (NSFC) [81171382, 31370822]
- National Key Basic Research (973) Program [2011CB707701]
- Beijing Natural Science Foundation (BNSF) [7142089]
- Tsinghua National Lab for Information Science and Technology (TNList) Cross-discipline Foundation
- Tsinghua-Peking Center for Life Sciences (CLS)
The polycystic TRP subfamily member PKD2-L1, in complex with PKD1-L3, is involved in physiological responses to diverse stimuli. A major challenge to understanding whether and how PKD2-L1/PKD1-L3 acts as a bona fide molecular transducer is that recombinant channels usually respond with small or undetectable currents. Here, we discover a type of Ca2+ influx-operated Ca2+ entry (ICE) that generates pronounced Ca2+ spikes. Triggered by rapid onset/offset of Ca2+, voltage, or acid stimuli, Ca2+-dependent activation amplifies a small Ca2+ influx via the channel. Ca2+ concurrently drives a self-limiting negative feedback (Ca2+-dependent inactivation) that is regulated by the Ca2+-binding EF hands of PKD2-L1. Our results suggest a biphasic ICE with opposite Ca2+ feedback regulation that facilitates sensory responses to multimodal transient stimuli. We suggest that such a mechanism may also occur for other sensory modalities and other Ca2+ channels.
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