期刊
CELL REPORTS
卷 10, 期 10, 页码 1639-1645出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.02.032
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资金
- NIH National Center for Research Resources (NCRR)
- European Molecular Biology Organization Long-Term Fellowship [ALTF 564-2010]
- NIH [R01 GM065591]
- Howard Hughes Medical Institute
The synaptonemal complex (SC) is a conserved protein complex that stabilizes interactions along homologous chromosomes (homologs) during meiosis. The SC regulates genetic exchanges between homologs, thereby enabling reductional division and the production of haploid gametes. Here, we directly observe SC assembly (synapsis) by optimizing methods for long-term fluorescence recording in C. elegans. We report that synapsis initiates independently on each chromosome pair at or near pairing centers-specialized regions required for homolog associations. Once initiated, the SC extends rapidly and mostly irreversibly to chromosome ends. Quantitation of SC initiation frequencies and extension rates reveals that initiation is a rate-limiting step in homolog interactions. Eliminating the dynein-driven chromosome movements that accompany synapsis severely retards SC extension, revealing a new role for these conserved motions. This work provides the first opportunity to directly observe and quantify key aspects of meiotic chromosome interactions and will enable future in vivo analysis of germline processes.
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