期刊
CELL REPORTS
卷 10, 期 6, 页码 1007-1019出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.01.022
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资金
- Agence Nationale de la Recherche [ANR-10-INSB-04-01]
- AFM (AFM-PainT)
- ERC [260435]
- European Research Council (ERC) [260435] Funding Source: European Research Council (ERC)
Cutaneous C-unmyelinated MRGPRD(+) free nerve endings and C-LTMRs innervating hair follicles convey two opposite aspects of touch sensation: a sensation of pain and a sensation of pleasant touch. The molecular mechanisms underlying these diametrically opposite functions are unknown. Here, we used a mouse model that genetically marks C-LTMRs and MRGPRD(+) neurons in combination with fluorescent cell surface labeling, flow cytometry, and RNA deep-sequencing technology (RNA-seq). Cluster analysis of RNA-seq profiles of the purified neuronal subsets revealed 486 and 549 genes differentially expressed in MRGPRD-expressing neurons and C-LTMRs, respectively. We validated 48 MRGPD- and 68 C-LTMRs-enriched genes using a triple-staining approach, and the Ca(v)3.3 channel, found to be exclusively expressed in C-LTMRs, was validated using electrophysiology. Our study greatly expands the molecular characterization of C-LTMRs and suggests that this particular population of neurons shares some molecular features with A beta and A delta low-threshold mechanoreceptors.
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