期刊
CELL REPORTS
卷 12, 期 6, 页码 1019-1031出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.07.003
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资金
- Association of International Cancer Research [08-0614, 10-0643]
- Academy of Finland [122546, 137687, 267817]
- Finnish Cancer Organisations
- Foundation of Finnish Cancer Institute
- Sigrid Juselius Foundation
- NIH [R01 CA129040, R01 CA100855]
- European Research Council (Coloncan)
- European Commission FP7-Health [278568]
- Cancer Research UK [A12481]
- Versus Arthritis
- Cancer Research UK [21139] Funding Source: researchfish
- Worldwide Cancer Research [10-0643] Funding Source: researchfish
- Academy of Finland (AKA) [267817, 267817, 122546] Funding Source: Academy of Finland (AKA)
An understanding of the mechanisms determining MYC's transcriptional and proliferation-promoting activities in vivo could facilitate approaches for MYC targeting. However, post-translational mechanisms that control MYC function in vivo are poorly understood. Here, we demonstrate that MYC phosphorylation at serine 62 enhances MYC accumulation on Lamin A/C-associated nuclear structures and that the protein phosphatase 2A (PP2A) inhibitor protein CIP2A is required for this process. CIP2A is also critical for serum-induced MYC phosphorylation and for MYC-elicited proliferation induction in vitro. Complementary transgenic approaches and an intestinal regeneration model further demonstrated the in vivo importance of CIP2A and serine 62 phosphorylation for MYC activity upon DNA damage. However, targeting of CIP2A did not influence the normal function of intestinal crypt cells. These data underline the importance of nuclear organization in the regulation of MYC phosphorylation, leading to an in vivo demonstration of a strategy for inhibiting MYC activity without detrimental physiological effects.
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