期刊
CELL REPORTS
卷 13, 期 10, 页码 2098-2106出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.11.011
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资金
- Humboldt-Universitat zu Berlin in the framework of the Excellence Initiative of the BMBF
- Deutsche Forschungsgemeinschaft (DFG)
- European Research Council [ERC-2010-StG-260590]
- European Union [323945]
- DFG [Exc 257, FOR 1341, FOR 2143]
- Helmholtz Society
Little is known about the properties of monosynaptic connections between identified neurons in vivo. We made multiple (two to four) two-photon targeted whole-cell recordings from neighboring layer 2 mouse somatosensory barrel cortex pyramidal neurons in vivo to investigate excitatory monosynaptic transmission in the hyperpolarized downstate. We report that pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. The majority of unitary excitatory postsynaptic potentials were small in amplitude (<0.5mV), with a small minority >1 mV. The coefficient of variation (CV = 0.74) could largely be explained by the presence of synaptic failures (22%). Both the CV and failure rates were reduced with increasing amplitude. The mean paired-pulse ratio was 1.15 and positively correlated with the CV. Our approach will help bridge the gap between connectivity and function and allow investigations into the impact of brain state on monosynaptic transmission and integration.
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