期刊
CELL AND BIOSCIENCE
卷 5, 期 -, 页码 -出版社
BMC
DOI: 10.1186/2045-3701-5-3
关键词
Human adipose tissue-derived stem cells; Advanced glycation end products; Mitogen-activated protein kinase; Apoptosis; Receptor for advanced glycation end products
资金
- National Basic Science and Development Program (973 Program) [2012CB518103]
- National Natural Science Foundation [81370883]
- Liaoning Province Science and Technology Plan [2011225020, 2012225080, 2012225014, 201322503]
- Shenyang Science and Technology Program [F11 262 9 01, F11 262 9 52]
Background: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation. Results: AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK. Conclusions: These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway.
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