期刊
STEROIDS
卷 78, 期 9, 页码 860-865出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.steroids.2013.05.008
关键词
Physalis pubescens L.; UPLC-ESI-MS analysis; Withanolides; Quantum chemical DFT-GIAO calculations; QR induction activity
资金
- National Scientific Foundation of China (NSFC) [31170325]
- Zhejiang Key Science & Technological Program [2009C13028]
In the present study, it was demonstrated that the dichloromethane extract of Physalis pubescens L (DEPP) had weak potential quinone reductase (QR) inducing activity, but an UPLC-ESI-MS method with glutathione (GSH) as the substrate revealed that the DEPP had electrophiles (with an alpha,beta-unsaturated ketone moiety). These electrophiles could induce quinone reductase (QR) activity, which might be attributed to the modification of the highly reactive cysteine residues in Keap1. Herein, four withanolides, including three new compounds physapubescin B (2), physapubescin C (3), physapubescin D (4), together with one known steroidal compound physapubescin (1) were isolated. Structures of these compounds were determined by spectroscopic analysis and that of physapubescin C (3) was confirmed by a combination of molecular modeling and quantum chemical DFT-GIAO calculations. Evaluation of the QR inducing activities of all withanolides indicated potent activities of compounds 1 and 2, which had a common alpha,beta-unsaturated ketone moiety. (C) 2013 Elsevier Ltd. All rights reserved.
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