期刊
STEROIDS
卷 74, 期 10-11, 页码 859-862出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.steroids.2009.05.007
关键词
Biotransformation; Microbial Baeyer-Villiger oxidation; DHEA; Pregnenolone; Steroidal lactones; Penicillium camemberti
Penicillium camemberti AM83 strain is able to carry out effective Baeyer-Villiger type oxidation of DHEA, pregnenolone, androstenedione and progesterone to testololactone. Pregnenolone and DHEA underwent oxidation to testololactone via two routes: through 4-en-3-ketones (progesterone and/or androstenedione respectively) or through 3 beta-hydroxy-17a-oxa-D-homo-androst-5-en-17-one. Analysis of transformation progress of studied substrates as function of time indicates that the 17 beta-side chain cleavage and oxidation of 17-ketones to D-lactones are catalyzed by two different, substrate-induced, BVMOs. In the presence of a C-21 substrate (pregnenolone or progesterone) induction of the enzyme catalyzing cleavage at 17 beta-acetyl chain was observed, whereas DHEA and androstenedione induced activity of the BVMO responsible for the ring-D oxidation; 5-en-3 beta-alcohol was a more effective inducer that the respective 4-en-3-ketone. (C) 2009 Elsevier Inc. All rights reserved.
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