期刊
STEROIDS
卷 73, 期 11, 页码 1187-1196出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.steroids.2008.05.009
关键词
11 beta-hydroxysteroid dehydrogenase; endothelial cells; kidney; vascular tissue; glucocorticoids
Vascular tissue expresses two isoforms of the enzyme 11 beta-Hydroxysteroid dehydrogenase, 11 beta-HSD1 and 11 beta-HSD2. These enzymes are responsible for the local metabolism of endogenous glucocorticoids (GCs). 11 beta-HSD1 deactivates GCs to their 11 keto metabolites or transforms inert 11 keto metabolites back to active GCs. Although, bi-directional, vascular 11 beta-HSD1 favors reactivation (reductase) over the deactivation (dehydrogenase) reaction, 11 beta-HSD2 only functions as a dehydrogenase. GC deactivation by enhanced 11 beta-HSD2 dehydrogenase activity or by impaired 11 beta-HSD1 reductase activity correlates with lower vascular resistance. These studies were designed to demonstrate the existence and regulation of these isoforms in vascular endothelial cells and to determine whether the expression varied by species and locale. Western blots were prepared from pre-confluent and confluent cultures of human umbilical vein endothelial cells (HUVEC). 11 beta-HSD1 was clearly expressed while 11 beta-HSD2 was much less prominent. Cultured rat aortic and bovine glomerular endothelial cells showed a similar pattern. Using immunohistochemistry, endothelial cells from human and mouse artery preparations clearly demonstrated 11 beta-HSD1. In separate experiments, pre-confluent growing HUVEC expressed more 11 beta-HSD1 compared to confluent cells. Serum-deprived growth-retarded HUVEC expressed significantly less 11 beta-HSD1. The enhanced expression of 11 beta-HSD1 was also observed 24h following a scratch injury to the culture plates. Changes in 11 beta-HSD1 with growth and during repair occurred at the transcription level. Thus, 11 beta-HSD1 protein expression predominates in endothelial cells and varies during periods of growth. (c) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据